What Were Looking For: We are looking for candidates with a biology or biotechnology. Penn Genomics Analysis Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute. Our primary product is Sequencher, DNA Sequence Analysis Software. In this tutorial you will become familiar with Sequencher’s sequence trimming functions, starting with vector trimming and then focusing on trimming intron sequence from amplified exons. Facility staff will also submit sequences to Genebank if requested by the investigator. Sequencher has a command for removing vector sequence, Sequence > Trim Vector, but this function is much more versatile than its name implies. Genomic sequences can also be submitted to the GAIA genome annotation system at the Bioinformatics Laboratory. Please follow the procedure listed below for installing Sequencher: Download and install the Sequencher program and the corresponding client appropriate for. Sequencher client licenses are available as. Inspired by our clients desire for custom features to help them with their projects, Sequencher has a wide range of features to help you get results from. Choose one computer to act as a Sequencher Server and then Sequencher client computers can check out licenses from the Server until all your licenses are in use. Database SearchesīLAST, BLOCKS, BLITZ, PROSITE and FASTA searches are performed using Genomic sequences can also be processed to identify interspersed repeats, low complexity regions, GRAIL exons, transcription factor binding sites, frequency of mono, di, and tri nucleotides, and other features. Sequencher Network Options: Sequencher License Server: Ideal for a single concurrent user environment or a 100 concurrent user environment.
Davison Life Sciences Bldg Room B118B 120 Green St. The network licenses of MacVector, Sequencher and other desktop software are available from the Bioinformatics group of the Penn Molecular Profiling Facility. If you experience problem using the license server replace it with 172.17.38.137 as the license server address. Training on the use of MacVector and Sequencher is provided free to the investigators. These services are included at no charge for large sequencing projects. Sequence Analysis/Database Searches Sequence Assembly & AnalysisĪssembly of sequences into a contig, identification of ORFs, analysis of properties of the predicted protein, and other analyses can be performed at the facility using MacVector, Sequencher and other software.
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